2100 Retriever: Antigen retrieval device for Immunohistochemistry
2100 Retriever has been designed to guarantee identical processing of all samples both in a single cycle and in independent sessions thanks to its automated system. Recover antigens from formalin-fixed, paraffin-embedded tissue simply and safely:
- Improves staining of formalin-fixed tissues.
- Handle up to 108 slides in a single session.
- Thermally processes paraffin-embedded tissue slides in just 30-40 minutes, ensuring high-quality immunostaining and reproducibility.
- It uses up to six different buffers simultaneously, essential to optimize epitope recovery.
- Preserves tissue morphology, even in delicate samples such as intestinal tissue.
- Prevents the formation of air bubbles thanks to precise pressure and temperature control.
Preserve tissue morphology and cell shape with the 2100 Retriever device
Thanks to its precisely calibrated pressure and heating cycle, the 2100 Antigen Retriever device effectively prevents boiling of the buffer in the chambers, thus preventing the formation of air bubbles that can negatively affect tissue morphology.
The images below compare (left) a sample of unprocessed intestinal tissue, (middle) tissue processed in a microwave (representing the standard result), (right) tissue processed with the Retriever.
This is a section of intestinal tissue fixed with formalin and embedded into paraffin.
Processing the tissue in microwave-type machines results in damage of the morphology of the gentle tissues.
No damage to morphology occurs, however, after processing the tissue in Retriever, although the unmasking is usually better than after the microwave.
More than 600 scientific references in journals such as:
Check out some research in cancer, cell biology, virology, immunology, neurosciences:
Chung, K.M., Singh, J., Lawres, L., Dorans, K.J., Garcia, C., Burkhardt, D.B., Robbins, R., Bhutkar, A., Cardone, R., Zhao, X., et al. (2020). Endocrine-Exocrine Signaling Drives Obesity-Associated Pancreatic Ductal Adenocarcinoma. Cell 181, 832-847.e18. 10.1016/j.cell.2020.03.062.
Zhou, D., Conrad, C., Xia, F., Park, J.-S., Payer, B., Yin, Y., Lauwers, G.Y., Thasler, W., Lee, J.T., Avruch, J., et al. (2009). Mst1 and Mst2 maintain hepatocyte quiescence and suppress hepatocellular carcinoma development through inactivation of the Yap1 oncogene. Cancer Cell 16, 425–438. 10.1016/j.ccr.2009.09.026.
Perera, R.M., Stoykova, S., Nicolay, B.N., Ross, K.N., Fitamant, J., Boukhali, M., Lengrand, J., Deshpande, V., Selig, M.K., Ferrone, C.R., et al. (2015). Transcriptional control of autophagy-lysosome function drives pancreatic cancer metabolism. Nature 524, 361–365. 10.1038/nature14587.
Gao, X., Vockley, C.M., Pauli, F., Newberry, K.M., Xue, Y., Randell, S.H., Reddy, T.E., and Hogan, B.L.M. (2013). Evidence for multiple roles for grainyhead-like 2 in the establishment and maintenance of human mucociliary airway epithelium. PNAS 110, 9356–9361. 10.1073/pnas.1307589110
Elgström, E., Ohlsson, T.G., and Eriksson, S.E. (2017). Cytokine evaluation in untreated and radioimmunotherapy-treated tumors in an immunocompetent rat model. Tumour Biol. 39, 1010428317697550. 10.1177/1010428317697550.
Epitope-specific recovery buffers
A comprehensive solution
Epitopes of varying nature may necessitate the use of different recovery buffers to achieve optimal results. In the case of formalin-fixed/paraffin-embedded (FF/PE) sections, the majority of commercial antibodies, which are typically designed against peptide epitopes or bacteria-expressed protein fragments, can be effectively unmasked using our R-Universal buffer.
However, we have discovered specific instances where, in addition to R-Universal, the use of alternative buffers yields even better outcomes. The choice of the appropriate buffer for epitope recovery depends on the epitope’s structural characteristics and the specific amino acids that compose it. Therefore, utilizing different buffers tailored to the epitope’s structure can enhance the quality of the recovery process.
