The Utility of CRISPR/CAS9 GFP System
Adding GFP to our experiment gives us the advantage of being able to select cells via fluorescence, separate them if we have a flow cytometer, and also be able to visualize the function of the protein as the cells differentiate and divide. The utility of CRISPR/CAS9...
Why is my Elisa test not working?
ELISA is a highly sensitive technique, since it is based on antigen-antibody specifity to determine the presence and quantity of a metabolite or antibodies. As a precise technique, small variations may lead to erroneous results. This is why one very common question...
How to choose competent cells
Competent cells are cells capable of absorbing endogenous DNA to introducing it into their interior. Once internalized, the DNA can recombine with its own genome, which is called horizontal transfer. Horizontal transfer is a mechanism of movement of genetic material...
