CRISPR experiment screening
CRISPR-Cas technology use is widespread in experimental and applied research due to its precision and ease of use for editing cells, tissues and whole organisms. In fact, CRISPR system has become the most favorite molecular biology laboratory genome editing tool....
CRISPR technology applications
From 2012 CRISPR-Cas system has become the most favorite molecular biology laboratory genome editing tool. This is due to its precision and ease of use for editing cells, tissues and whole organisms. Thus, CRISPR-Cas technology use is widespread in experimental and...
How to avoid off-target events in crispr experiments
CRISPR technology has contributed in recent years to the revolution of genetic engineering, allowing genes to be modified in a simple way. This system is composed of 2 key elements: the RNA guide that binds to the target DNA, to direct the second component, Cas9,...
How to design guides for CRISPR
The CRISPR system has revolutionized the field of genetic engineering. It allows scientist to alter genes in a simple way. The basic principle of CRISPR is based on cutting at a specific point the target DNA, using a nicase enzyme (Cas) which is guided by a fragment...
