5 methods to quantify proteins
The precise quantification of the proteins in a sample is essential for their study in an infinite number of research fields. While the quantification of a specific protein can be carried out using assays such as Western Blot or ELISA, by mass spectrometry (MS) or by...
Enzymes for research: types and applications
Enzymes are organic molecules that act as catalysts for chemical reactions. Due to its functional diversity, they are one of the most widely used biomolecules in scientific research laboratories. These catalytic proteins play a fundamental role in areas as diverse as...
The Utility of CRISPR/CAS9 GFP System
Adding GFP to our experiment gives us the advantage of being able to select cells via fluorescence, separate them if we have a flow cytometer, and also be able to visualize the function of the protein as the cells differentiate and divide. The utility of CRISPR/CAS9...
Why is my Elisa test not working?
ELISA is a highly sensitive technique, since it is based on antigen-antibody specifity to determine the presence and quantity of a metabolite or antibodies. As a precise technique, small variations may lead to erroneous results. This is why one very common question...
