CRISPR, advanced genome engineering at your fingertips
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CRISPR technology is widely used in labs and not just for genetic edition.
Everyday CRISPR technology is improving and thus its uses are expanded.
In this article we will review some of these uses such as the visualization of endogenous genomic elements in living cells through imaging and detection systems as well as the most widespread applications of genome editing and regulation.
Off-target effects in CRISPR experiments are cuts introduced by Cas enzyme in DNA locations other than the specific target.
Discover the key aspects to design the RNA guides for CRISPR, which direct the Cas enzyme to the DNA cutting site.