This Western-Blot protocol wants to be an easy and basic guide for researchers to help them through the process. It contains a list of materials, the steps to follow and even a list of common problems and mistakes that might arise and their solutions.
A western blot is a laboratory method used to detect specific protein molecules from among a mixture of proteins. This mixture can include all the proteins associated with a particular tissue or cell type. Western blots can also be used to evaluate the size of a protein of interest, and to measure the amount of protein expression.
This Western-Blot protocol contains:
- Materials and reactives
- Gel preparation
- Sample loading
- Gel transfer
- Immunoblotting
- Troubleshooting
Related content
Want to know more? Check some of these articles related to the topic.
5 Methods to quantify proteins
About Abyntek
At Abyntek Biopharma we have more than 15 years of experience giving the best possible service to researchers. That’s why we put all our know-how and experience both into helping researchers to find the perfect reagent for their research. And why we have put all the experience of our scientific team to create support documents such as these to guide and help researchers on their day to day.
