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Protein

Recombinant Rat Vascular Endothelial Growth Factor 164, Yeast

Consultar

DESCRIPCIÓN

Supplier:Signalway
Reference:AP60253
Name:Recombinant Rat Vascular Endothelial Growth Factor 164, Yeast
Category:Protein
Subcategory:Proteins with activity
Unit:10ug 100ug 500ug
price :Consultar
format :Lyophilized from a 0.2 ?m filtered concentrated solution in PBS, pH 7.4.
purification :> 95 % by SDS-PAGE and 90% by SEC-HPLC analyses.
Storage conditions :Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 ? as supplied.1 month, 2 to 8 ? under sterile conditions after reconstitution.3 months, -20 to -70 ? under sterile conditions after reconstitutio
Aa sequence :MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
endotoxin :Less than 0.1 EU/ug of rRtVEGF164, Yeast as determined by LAL method.
Molecular weight :Theoretically as a disulfide-linked homodimeric protein, the product consists of two 165 amino acid polypeptide chains. As a result of glycosylation, it migrates to at least two bands with molecular weights ranging from 25.7 kDa in SDS-PAGE under reducing
bioactivity :Fully biologically active when compared to standard. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.75-3.75 ng/ml.


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