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P

Protein

Recombinant Human Malic Enzyme 2, His

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DESCRIPCIÓN

Supplier:Signalway
Reference:AP60410
Name:Recombinant Human Malic Enzyme 2, His
Category:Protein
Subcategory:Proteins with activity
Aditional Names:NAD-dependent Malic Enzyme, Mitochondrial, NAD-ME
Unit:5ug 100ug 500ug
price :Consultar
format :Lyophilized from a 0.2 ?m filtered concentrated solution in PBS, pH 7.4.
purification :> 95 % by SDS-PAGE and HPLC analyses.
Storage conditions :Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ? as supplied.- 1 month, 2 to 8 ? under sterile conditions after reconstitution.- 3 months, -20 to -70 ? under sterile conditions after reconst
Aa sequence :MLHIKEKGKP LMLNPRTNKG MAFTLQERQM LGLQGLLPPK IETQDIQALR FHRNLKKMTS PLEKYIYIMG IQERNEKLFY RILQDDIESL MPIVYTPTVG LACSQYGHIF RRPKGLFISI SDRGHVRSIV DNWPENHVKA VVVTDGERIL GLGDLGVYGM GIPVGKLCLY TACAGIRPDR CLPVCIDVGT DNIALLKDPF YMGLYQKRDR TQQYDDLIDE FMKAITDRYG RN
endotoxin :Less than 1 EU/ugof rHuME2, His as determined by LAL method.
Molecular weight :Approximately 64.4 kDa, a single non-glycosylated polypeptide chain containing 573 amino acids with 6 x His at C-terminus.
bioactivity :Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer (1975). The standard reaction mixture contained 50 mM Tris-HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate. Assay was performed in a Beckman s
Ncbi gene id :4200
Uniprot number :P23368


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