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Protein

Recombinant Bifunctional ligase/repressor BirA, His

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DESCRIPCIÓN

Supplier:Signalway
Reference:AP60432
Name:Recombinant Bifunctional ligase/repressor BirA, His
Category:Protein
Subcategory:Proteins with activity
Unit:5ug 100ug 500ug
price :Consultar
format :Supplied as a 0.2 ?m filtered solution in 50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 10 % glycerol.
purification :> 95 % by SDS-PAGE analyses.
Storage conditions :Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 ? as supplied.- 3 months, -20 to -70 ? under sterile conditions after opening.
Aa sequence :MKDNTVPLKL IALLANGEFH SGEQLGETLG MSRAAINKHI QTLRDWGVDV FTVPGKGYSL PEPIQLLNAK QILGQLDGGS VAVLPVIDST NQYLLDRIGE LKSGDACIAE YQQAGRGRRG RKWFSPFGAN LYLSMFWRLE QGPAAAIGLS LVIGIVMAEV LRKLGADKVR VKWPNDLYLQ DRKLAGILVE LTGKTGDAAQ IVIGAGINMA MRRVEESVVN QGWITLQEAG IN
endotoxin :Less than 0.1 EU/ugof rBirA, His as determined by LAL method.
Molecular weight :Approximately 36.4 kDa, a single non-glycosylated polypeptide chain containing 329 amino acids, with 6 x His at the C-terminus.
bioactivity :Measured by its ability to generate pyrophosphate from the biotinylation reaction. The pyrophosphate is subsequently hydrolyzed using Recombinant Yeast Inorganic Pyrophosphatase/PPA1 (ryPPA1). The specific activity is > 10.0 pmol/min/?g, as measured under


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