This Dot-Blot protocol wants to be an easy and basic guide for researchers to help them through the process. It contains a list of materials, the steps to follow and even a list of common problems and mistakes that might arise, as well as their solutions.
Dot-Blot is a simple immunological technique for detecting the presence of biomolecules. It is used to detect DNA, RNA, or proteins. One of the advantages of this technique is that the sample is added directly to the membrane, thus allowing us to save time.
However, it only detects the presence or absence of the target molecule, since it does not allow the molecules to be separated and, therefore, differentiated by their size.
This Dot-Blot protocol contains
- Materials and reactives
- How to reduce mistakes?
At Abyntek Biopharma we have more than 15 years of experience giving the best possible service to researchers. That’s why we put all our know-how and experience both into helping researchers to find the perfect reagent for their research. And why we have put all the experience of our scientific team to create support documents such as these to guide and help researchers on their day to day.