This immunocytochemistry protocol describes the steps for performing the immunocitochemistry method on samples of intact cells that have had most, if not all, of their surrounding extracellular matrix removed.
Immunocytochemistry (ICC) is a technique used to assess the presence of a specific protein or antigen in intact individual cells (eg. cultured cells, cell suspensions) by use of a specific antibody that binds to it. ICC is a valuable tool to study the presence and sub-cellular localization of proteins. This immunochemistry technique is performed on samples of intact cells that have had most, if not all, of their surrounding extracellular matrix removed.
This protocol describes the steps for performing the ICC method. Please be aware that the protocol may need to be optimized for different cells, products, target proteins, etc.
This immunocytochemistry protocol contains:
- Materials and reactives
- Slide preparation
- Fixation
- Antigen retrival
- Permeabilization
- Blocking
- Immunostaining
- Counterstaining
- Mounting
- Troubleshooting
Related content
The complete guide to Inmunohistochemistry
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